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Vitamin B12 is water-soluble, meaning it dissolves in water and passes through the body quickly so must be replenished regularly by eating foods that contain it or by taking a dietary supplement if necessary

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Synthesis of sumyolated H4 Purified SUMO-3(2–91)C47S-MESNa thioester (1 eq.) and H4(1–14) aux -C(O)NHNH 2 (6 eq.) were reacted in a buffer containing 6 M Gn-HCl, 100 mM Na 2 HPO 4 , and 10 mM TCEP, pH 7.3