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In vivo, hepatic knockdown of GADD45β in MCD-fed mice markedly reduced SIRT1 expression and the p-AMPK/AMPK ratio as shown by both western blotting and immunohistochemistry (Fig

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2.1 Precise Control of Core Fermentation Parameters We have established optimal control ranges for key fermentation parameters, laying a solid foundation for stable, large-scale production: · Carbon source concentration (glucose): 22–28 g/L · Magnesium ion concentration (magnesium sulfate): 2.0–3.0 g/L · Culture temperature: 26–30°C Through continuous fermentation monitoring, we determined a 24-hour cycle as the optimal fermentation period