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Membranes were washed several times with Tris-buffered saline, 0.1% Tween 20 (TBST) and incubated with anti-rabbit (RRID: AB_631746, Santa Cruz Biotechnology, In, 1:5000), anti-mouse (RRID: AB_2687626, Santa Cruz Biotechnology, In, 1:5000) or anti-goat (RRID: AB_628490, Santa Cruz Biotechnology, In, 1:2000) horseradish peroxidase-linked immunoglobulin G for 2 h at room temperature, and the membranes were washed several times with TBST

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Finally, by in situ hybridization and immunostaining, BPC 157 was found in human fetus and adult gastrointestinal mucosa, lung bronchial epithelium, epidermal layer of the skin and kidney glomeruli [1,9]

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