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ROS act as key secondary messengers in cells, influencing various cellular functions, including regulating gene expression, controlling cell survival, and modulating intracellular signaling networks ( 2.2.1 Oxidative stress and bone formation Hydrogen peroxide (H 2 O 2 ) inhibit osteoblast proliferation, whereas the antioxidant N-acetylcysteine (NAC) significantly restores it ( Cell proliferation is controlled by a crucial signaling protein called mammalian target of rapamycin (mTOR)

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