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Usually, that means methionine, inositol, choline, and B12 together—the classic “MIC-B12” shot

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Treatments with palmitate (PA, 100 µM) NAD + (50 µM), NMN (100 µM), EX527 (5 µM) and/or 78c (3 µM) were added and cultures were allowed to differentiate for 72 hr