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Therefore, it is plausible that hydrochlorothiazide, by targeting carbonic anhydrases and other enzymes, may have wider metabolic effects than predicted, at least in animal models

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The resulting mixture is electroporated with 200,000 cells in electroporation buffer and the cells are deposited into antibiotic-free medium, as described above, and allowed to rest in a 37 °C incubator for 24 h before additional antibiotic-containing medium was added