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3.2 Colorimetric and fluorometric assays One of the most popular colorimetric methods for GSNO detection is the Saville assay which relies on cleavage of the S-NO bond by mercury(II) [35] followed by reaction of liberated nitrite with N-(1-naphtyl)ethylenediamine dihydrochloride in acidic media to form the colored adduct in the Griess reaction [36]
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