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Using the Illumina Stranded mRNA Sample Prep Kit (protocol version: # 1000000124518 v03, June 2022), poly-A containing mRNA molecules were purified from the total RNA input using poly-dT oligo-attached magnetic beads
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Czeiter E, Amrein K, Gravesteijn BY, Lecky F, Menon DK, Mondello S, Newcombe VFJ, Richter S, Steyerberg EW, Vyvere TV, Verheyden J, Xu H, Yang Z, Maas AIR, Wang KKW, Büki A (2020) Blood biomarkers on admission in acute traumatic brain injury: Relations to severity, CT findings and care path in the CENTER-TBI study