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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed with the PowerUp SYBR Green Master (Applied Biosystems) on a 7500 Fast thermocycler (Applied Biosystems)

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FIGURE 5 Ferroptosis, a regulatory cell death mode, is characterized by iron-dependent and lipid peroxide (LPO) accumulation, proposed by 2+ , which can produce •OH through the Fenton reaction, and then react with polyunsaturated fatty acids in the plasma membrane to produce ROS, resulting in iron-regulated death