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Although they share a high degree of homology, the physiologic roles of these hormones are unique, governed by differences in expression pattern, biopotency, and regulation — with LH serving as a key regulator of gonadal steroidogenesis and ovulation, and hCG predominantly active in pregnancy and fetal development

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Cells were harvested using radioimmunoprecipitation assay lysis buffer supplemented with phosphatase inhibitor cocktails 1 and 2 (Sigma-Aldrich)