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Based on preclinical (animal and cellular) data only, inhibition of NNMT by 5-Amino-1MQ is theorised to: Raise intracellular NAD+ levels Activate SIRT1 and other sirtuins (proteins linked to metabolic regulation) Reduce fat cell (adipocyte) size and proliferation Improve insulin sensitivity These effects have been demonstrated in mouse models (Neelakantan et al., 2019), but robust human clinical trial data are currently absent

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Indeed, PLIN1 phosphorylation is required for PKA-dependent lipolysis (44, 93) by promoting the release of ABHD5 and interaction with PNPLA2 in response to PKA (12, 16) and for proper LD recruitment and activation of HSL (44)