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Determination of optimal drug concentrations for CRISPR screen HEK293 cells were seeded in 6-well plates at a density optimized to remain below 90% confluency after 72 h of treatment with minimal concentrations of drugs either inhibiting mitochondrial translation (chloramphenicol) or inducing mitochondrial mistranslation (actinonin)

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BPC-157 may potentiate migration and the spreading of tendon fibroblasts [11]