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Glutathione Sepharose beads (40 μL per tube) were washed three times with ultrapure water (500 μL) and equilibrated twice with equilibration buffer (50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1% NP-40, 1 mM DTT, 10 mM MgCl 2 , pH 8.0)