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They were next embedded in paraffin routinely prepared to be 5–6 microns thick and were stained with hematoxylin and eosin (H and E) for light microscopy.[18] The histopathological assessment of ulceration in the colon samples was scored based on the inflammatory severity and extent, crypt damage, and leukocyte infiltration, and finally, total colitis index was calculated based on these parameters as described in previous works.[19] Determination of myeloperoxidase (MPO) activity in colon tissue First, 100 mg of the tissue was weighed and completely crushed
