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Soluble lysates (~900 μg protein) were precleaned with protein A-Sepharose beads (Amersham Pharmacia Biotech, Piscataway, NJ) at 4˚C for 30 min on a rotator, and then incubated with two rabbit polyclonal Abs against Mkp-1 (4 μg each, Mkp-1 (V15) and Mkp-1 (M18)) and protein A-Sepharose beads (25 μl) overnight at 4˚C on a rotator
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Size:10 ml.
Survodutide , a dual GLP-1/glucagon receptor agonist from Boehringer Ingelheim, demonstrated 18.7% weight loss at 46 weeks in Phase II (530 patients)
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