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It has been described for roGFP1-iL that the insertion C147CL and the substitution H148S lower the thermodynamic stability of the inter-strand disulfide C147–C204 resulting in a less negative E °′ of the sensor (Lohman and Remington, E °′ roGFP2-iL was determined through titration with LA/DHLA

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Q5: How is Pinealon reconstituted