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Importantly, the proteomic similarity of Day 30 iHLOs to PHHs, with a 33.46% expression difference from liver tissue, underscores their physiological relevance compared to traditional models like HepG2 cells, which exhibit a 54.36% protein difference from liver tissue, or HepaRG cells, with a 45.40% protein difference

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These proteins consist of transmembrane (occludin, claudin, junctional adhesion molecules, and tricellulin) and cytoplasmic proteins (zonula occludens, cingulin, and afadin), which help coordinate the alteration of the cytoskeleton (23)