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Fractions containing Nɛ-Hcy-Lys isopeptide, a predominant product eluting at 2 min, were collected and the solvent was evaporated under a vacuum to leave a white powder

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Accumulating evidence suggests that the activation of PSCs and the development of CP involve several important signaling pathways, including Smad, PI3K-AKT, MAPK, and p38-MAPK [26] (Figure 2)