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The membrane was blocked using 5% (w/v) dried milk powder in Tris-buffered saline (TBS) [10 mM Tris-HCl (pH 7.5) and 0.15 M NaCl] at room temperature and incubated with primary antibodies raised against antigens of human origin for 2 h in TBS [rabbit polyclonal SOD-1 and GS (GSH2) antibodies from Santa Cruz Biotechnology, Inc