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This hypothesis was confirmed by several more recent trials with sublingual or oral doses of cobalamin between 1000 and 5000 µg [5–9]

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Cycling conditions were as follows: 50 °C and 95 °C both for 2 minutes each prior to 40 cycles of 95 °C 15 seconds denaturation, 60 °C 30 seconds annealing and 72 °C also 30 seconds for primer extension, generating a 193 or a 195 bp amplicon for Enterobacteriaceae or 16S rRNA gene, respectively