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Apoptosis assay Cells were collected from 24-well plates, spun down and incubated in darkness at 4 °C with 0.5% annexin-V-APC antibody (BD Pharmigen) and 100 μg ml −1 propidium iodide dissolved in annexin-V binding buffer (1 M CaCl, 5 M NaCl, 1 M HEPES)
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