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Because an internal reference can interfere with the acid-base equilibrium, an external reference is recommended, either with an extra measurement or a second co-axial sample tube

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A 5 to 8 minute plasma half-life means that by the time subcutaneously injected DSIP is absorbed from the injection site, cleared through lymphatics into venous blood, and circulated, a very substantial fraction has already been enzymatically degraded

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