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In this stage, two different catalysts would be produced (Table 1) (Kawai et al., 2019): the surface modification enzymes (associated with enzymatic modification of PET to increase the surface hydrophilicity) and the PET-degrading enzymes (capable of directly breaking the ester bonds within the PET polymer releasing monomeric products)
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This experimentation led to an upregulation of neurogenic differentiation markers, including Nestin, GAP43, β Tubulin III, and Doublecortin, in hGMSCs
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