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Next, we confirmed that similar to CRPC [18], in NEPC cells (H660), diminished tetrahydrobiopterin to the 7,8-dihydrobiopterin ratio (BH4:BH2) ratio led to the destabilization of NOS subunits (uncoupling) and shift the enzymatic activity towards superoxide (O2-) generation [28,29,30,31,32]

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