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treatment of the adipocytes with the NNMT inhibitor 5-amino-1MQ resulted in a concentration-dependent increase in the NAD+ levels with concentrations in the range of 1-60 μM resulting in ˜1.2-1.6-fold increase in NAD+ levels relative to control adipocytes
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High-dose biotin, also known as vitamin B7, can affect assays that use a biotin and streptavidin binding step, and the direction of the error depends on the assay format, with competitive designs prone to falsely high results and sandwich designs to falsely low ones
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